Fig. 8.

Eact depolarizes airway smooth muscle (ASM) cells. A: representative tracings of FLIPR membrane potentiometric dye demonstrating depolarization [increase in relative fluorescence units (RFU)] and hyperpolarization (decrease in RFU). Benz, benzbromarone. B: 25 μM Eact caused an acute depolarization compared with DMSO vehicle (−3.1 ± 3.0 RFU for DMSO control vs. 182.2 ± 28.5 RFU for 25 μM Eact, n = 14, ***P < 0.001). Pretreatment with the TMEM16A antagonist benzbromarone (18.3 ± 1.7 RFU, n = 8, P < 0.001) or the L-type voltage-dependent calcium channel blocker nifedipine (76.8 ± 14.0 RFU, n = 19, **P < 0.01) completely attenuated this depolarization. One-way ANOVA with post hoc Bonferroni’s test, *P < 0.05.