Fig. 2.

RT-PCR of ecto-nucleoside triphosphate diphosphohydrolase (ENTPDs) and nonspecific alkaline phosphatase (NSAP) in human bronchial epithelial cells (HBEC). mRNA quantification was performed in HBEC cultures from four non-cystic fibrosis (CF) (control, CTL) and four CF donors. Values were log transformed to achieve normality, and mean differences between CF and non-CF HBEC data were assessed with linear mixed models, with a sample as random effect factor (R package, nlme). The data were plotted using the 2^{(−∆∆CT)} transformation [CT, threshold cycles (33)]. The mean value for CTL ENTPD1 expression was used as relative baseline value. ENTPD3 and NSAP were significantly different from ENTPD1 and ENTPD2. *P < 0.05; **P < 0.001; ***P < 0.0001.