Figure 3.

Large-scale DNA assembly. Four L3 parts were assembled in the absence of a receiver plasmid through a SapI-mediated Loop assembly reaction and products were analyzed by pulsed-field gel electrophoresis. Lane headings: M, Midrange PFG marker. C, control reaction (L3 parts digested with SapI). Assembly reaction using 1× (10 U µl⁻¹) or 2× (20 U µl⁻¹) T4 DNA ligase. Image shown corresponds to an inverted photograph of the gel with adjusted contrast. The white arrow indicates the monomeric fragments, the blue arrow indicates the dimeric composites, the red arrow indicates the trimeric composites and the green arrow indicates the tetrameric full-length assembly.