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. 2020 Feb 5;5(1):ysaa001. doi: 10.1093/synbio/ysaa001

Figure 4.

Figure 4.

Performance of uLoop plasmids in E. coli. (A) Plate fluorometry of sfGFP/mRFP1 ratio of expression in E. coli. Performance of L1, L2 and L3 assemblies for all vector kits was assessed by measuring the ratio of expression of one copy of sfGFP per plasmid to a chromosomal mRFP1 cassette. These values were calculated from readings obtained over the full growth of cultures (see Supplementary Text). (B) Plasmid performance in E. coli measured by flow cytometry. Performance of L1, L2 and L3 assemblies for all vector kits was assessed by measuring the ratio of mean population values for green fluorescence (ex. 488 nm, em. 530 ± 15 nm) to red fluorescence (ex. 488 nm, em. 616 ± 11.5 nm) of cells expressing one copy of plasmid-borne sfGFP per plasmid and a chromosomal mRFP1 cassette. (C) Growth rates of L1, L2 and L3 assemblies for all vector kits and control AKR1 cells. Dots, boxes and lines correspond to three measurements performed on different days; boxes and lines show standard error of the mean and mean, respectively.