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. 2020 Feb 5;5(1):ysaa001. doi: 10.1093/synbio/ysaa001

Table 1.

Assembly integrity

Set Level Correct (n) Incorrect (n) Percentage (%)
pCA L1 7 1 87.5
L2 8 0 100
L3 6 2 75
L3* 7 1 87.5
pCO L1 7 1 87.5
L2 8 0 100
L3 5 3 62.5
L3* 5 3 62.5
pSB L1 8 0 100
L2 8 0 100
L3 5 3 62.5
L3* 4 4 50
pAN L1 8 0 100
L2 8 0 100
L3 7 1 87.5
L3* 8 0 100

Integrity of assembly using BsaI-HFv2 instead of BsaI.