Table 2.
Stability of uLoop plasmid in P. tricornutum
| Sub-strain—exconjugate number | % Blue fluorescent |
Fluorescence geom. mean |
Fluorescence CV (%) |
|||
|---|---|---|---|---|---|---|
| Month 1 | Month 4 | Month 1 | Month 4 | Month 1 | Month 4 | |
| CCMP632-1 | 67.1 | 62.5 | 9.62 | 5.97 | 150 | 131 |
| CCMP632-1-sort A | 99.5 | 36.1 | 72 | |||
| CCMP632-1-sort B | 97.1 | 28.7 | 57 | |||
| CCMP632-2 | 39.3 | 88.9 | 7.61 | 18.5 | 1516 | 77 |
| CCMP632-3 | 40.1 | 31.9 | 5.56 | 1.85 | 242 | 432 |
| CCMP632-4 | 34.5 | 59.2 | 4.57 | 8.64 | 1426 | 481 |
| CCMP632-5 | 56.2 | 57.6 | 10.9 | 6.11 | 328 | 321 |
| CCMP632-6 | 97.4 | 99.4 | 33.1 | 24.5 | 88 | 75 |
| RCC2967-1 | 48.6 | 49 | 4.82 | 3.5 | 474 | 1031 |
| RCC2967-1-sort A | 99.4 | 11.2 | 47 | |||
| RCC2967-1-sort B | 99.6 | 13.4 | 59 | |||
| RCC2967-2 | 93.9 | 97.8 | 16.3 | 10.2 | 56 | 40 |
| RCC2967-3 | 35.9 | 60.2 | 4.95 | 4.31 | 1629 | 140 |
| RCC2967-4 | 89.9 | 90.6 | 23.6 | 15.4 | 72 | 44.4 |
| RCC2967-5 | 25.9 | 51.1 | 3.37 | 4.63 | 608 | 444 |
Fluorescent phenotypes of 11 pSBL2-1_Pt-B exconjugate colonies from conjugations of two separate sub-strains (CCMP632 and RCC2967) were tested 3 months apart. The highest sub-populations of blue-fluorescent cells were sorted on two independent days at month 2.5 (sort A and sort B) from one exconjugate colony of each conjugation and re-tested at month 4 (see Supplementary Figure S17). Reported are the % of cells showing blue fluorescence above the 95th percentile for control (non-BFP) exconjugates, the geometric mean of blue fluorescence (normalized to control exconjugates), and the CV of blue fluorescence within each exconjugate. Fluorescence was measured logarithmically.