Table 2. Main tests reported in the studies with non-neuronal cells cultures.

Authors, Year	Cell Culture	Study Objectives	Cell Viability	Gene Expression	Others
Craenenbroeck et al., 2006	HEK293rtTA	To evaluate the effect of pipamperone on D4 receptor expression	-	qPCR	Radioligant Western blot
Wakamatsu et al., 2009	HEK 293	To evalute the effect of methylphenidate on the cardiovascular system	-	-	Electro-physiological
Ludolph et al., 2010	TsA201	To evaluate the effect of atomoxetine on glutamate receptors	-	-	Electro-physiological
Knorle et al., 2012	JAR	To evaluate the effect of Sideritis scardica on monoamine transporters	LDH	-	Effect on transporters
Suter et al., 2006	Human lymphocytes	To evaluate the clastogenic effect of methylphenidate	-	-	Chromosome aberration
Schwarz et al., 2014	Lymphoblastoid Cell Lines	To evaluate the effect of methylphenidate on gene expression regulation	-	qRT-PCR Microarray	-
Kittel-Schneider et al., 2016	Peripheral blood mononuclear cells	To evaluate the cytogenetic effects of long-term treatment with methylphenidate	-	-	Micronucleus
Craenenbroeck et al., 2006	L929sA	To evaluate the effect of pipamperone on D4 receptor expression		qPCR	Radioligant Western blot
Craenenbroeck et al., 2006	CHO	To evaluate the effect of pipamperone on D4 receptor expression	-	qPCR	Radioligant Western blot
Zhao et al., 2007	CHO	To evaluate the effect of Fructus Psoraleae on noradrenaline and dopamine transporters	-	-	Effect on transporters
Salviano et al., 2015	MDCK	Evaluate the effect of methylphenidate on the renal system	MTT	-	-

Abbreviations: HEK293rtTA cells: Human embryonic kidney cells expressing the tetracycline transactivator HEK-293: Human Embryonic Kidney; TsA201: Transformed human embryonic kidney; JAR: Human choriocarcinoma; L929sA: Cells mouse fibrosarcoma; CHO: Chinese Hamster Ovary; MDCK: Madin-Darby Canine Kidney; Receptor D4: Receptor de dopamine 4; 6-OHDA: 6-hydroxydopamine; qRT-PCR: quantitative Real Time PCR; *Eicosapentaenoic acid e docosahexanoic acid e gamma-linolenic acid.