| Identification of culture type |
|
| Identification of cell type |
|
| Origin of cells (human, animal) |
|
| In case of cells isolated from tissues, report isolation technique and variables involved |
|
| Source of access of cells (collection of cell biology, donation by laboratories) |
|
| In case of collection of cell biology, report the product/catalog number |
|
| Growth medium used |
|
| Growth medium supplementation components (serum, antibiotics, micronutrients, others). Report name, concentration, percentage used and brand |
|
| Frequency of change of growth medium |
|
| pH of the growth medium |
|
| Confluence |
|
| Use of trypsin or other substance to collect the cells. Report name, concentration, percentage |
|
| Cellular density |
|
| Number of passages |
|
| Incubation temperature (exact 0.0 °C) |
|
| Atmosphere conditions (exact 0.0% CO2) |
|
|
METHODS
|
| Technique name |
|
| Report whether the technique has been developed and validated or reproduced |
|
| In case of reproduced technique, report if there were adaptations and what were they |
|
| In case of use of substance that promotes cellular alteration, report name, function, concentration, percentage, exposure time |
|
| Time period of each step |
|
| Temperature (exact 0.0 °C) |
|
| Atmosphere conditions (exact 0.0% CO2) |
|
| Equipment used |
|
| Type of statistical analysis and level of significance |
|
| Software for statistical analysis |
|
