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. 2020 Jan 17;11(7):1780–1791. doi: 10.7150/jca.38880

Figure 3.

Figure 3

ASK induces cell death of BCa cell lines. UMUC-3, RT4 and T24 cells were transfected for 48 h with Andes-1537 or ASO-C or left untreated (NT). (A) Andes-1537 caused massive cell detachment from the substrate, as opposed to controls (NT, ASO-C) (Bars = 50 μm). (B, C) Andes-1537 induced 50-60% death in all 3 cell lines, compared to controls, as evidenced by Tb (B) and PI (C) exclusion. *p<0.05, **p<0.01, Andes-1537 vs. ASO-C. (D) Normal bladder epithelial cells were transfected with Andes-1537 or ASO-C or left untreated for 48 h and permeability to PI was determined by cytometry. Andes-1537 treatment displayed no significant level of cell death, like controls. Staurosporine (STP) treatment was used as positive control. **p<0.01, STP vs. Andes-1537.