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. 2020 Jan 17;11(7):1780–1791. doi: 10.7150/jca.38880

Figure 4.

Figure 4

ASK induces dissipation of mitochondrial membrane potential (Δψm). UMUC-3, RT4 and T24 cells treated with Andes-1537 or ASO-C, or left untreated (NT) for 24h or with uncoupling drug CCCP were harvested at 24 h post-transfection, stained with 20 nM TMRM for 15 min and analyzed by flow cytometry, revealing that ASK induces dissipation of Δψm. (B) A triplicate analysis shows that Andes-1537 induced between 50 and 80% dissipation of Δψm (UMUC-3, **p=0.0073; RT4, **p=0.0024; T24, p=0.017). (C) RT-PCR of total RNA purified from cells treated as in (A) for 48 h revealed the specific knockdown effect of Andes-1537 on ASncmtRNA-1 (AS-1) and ASncmtRNA-2 (AS-2). 18S rRNA was used as internal control. (M, 100-bp ladder).