Figure 8.

ASK delays BCa tumor growth in vivo. (A) Mice bearing tumors between 50-80 mm³ were randomized into 2 groups of 5 mice each, which were treated with Andes-1537 or saline alone. At 32 days post-cell injection, ASK induced a drastic inhibition of tumor growth (**p=0.0031), mice were euthanized under anesthesia and tumors were collected from both groups. (B) ASK induces TUNEL-positive cells in tumors. Only tumors from Andes-1537-treated mice showed positive TUNEL nuclear staining (Bars = 250 µm). Inserts show higher magnification of the same samples. (C) Andes-1537S inhibits growth of a BCa PDX tumor. On day 23 post-implantation, tumors of about 100 mm3 were observed and mice were randomized into two groups. The control group was injected ip with 200 µl saline and the treatment group with 100 µg Andes-1537. The remaining 9 injections of the treatment group were performed with 50 µg Andes-1537 (arrows). ASK induced significant inhibition of tumor growth, compared to saline (*p=0.0078). (D) At day 62 post-implantation, mice were euthanized under anesthesia and tumors sections were analyzed by TUNEL assay, showing that tumors from Andes-1537-treated PDX mice displayed positive nuclear signal (Bars = 250 µm).