Figure 4.

OA^nano hinders migration and invasion abilities of U87 glioma cells. A. U87 glioma cells observed under a phase contrast microscope after scratched and treated with DMSO or OA^nano for 24 h. The line confines the initial area of removed cells. bar=100 μm. B. The proportion of migrated U87 glioma cells after scratched and treated with DMSO or OA^nano for 24 h. C. The proportion of Vimentin positive U87 cells after treated with DMSO or OA^nano for 24 h. D. IHC analysis of Vimentin in U87 glioma cells after 24 h of treatment with DMSO or OA^nano. bar=50 μm. E. RT-PCR analysis of MMP-7 in U87 glioma cells treated with DMSO or OA^nano for 24 h. F. U87 glioma cells which remained on the upper layer of the Transwell chamber after treated with DMSO or OA^nano for 24 h. bar=100 μm. G. The proportion of U87 glioma cells remaining on the upper membrane of the Transwell chamber after treating with DMSO or OA^nano for 24 h. H. mRNA expression of MMP-7 in U87 glioma cells after treated with DMSO or OA^nano for 24 h.