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. 2020 Jan 14;11(7):1657–1667. doi: 10.7150/jca.38721

Figure 5.

Figure 5

Functional status of NFκB signaling. A, IkB phosphorylation following TNFα treatment. Cells were treated with 100ng/ml TNFα for times indicated in figure. Following TNFα treatment, cells were collected, lysed and total protein probed for pIkB. Beta-actin served as a loading control. Immunoblot is representative of immunoblots from three independent experiments. B, Translocation of p50 and p65 to the nucleus following TNFα treatment. Cells were treated with 100ng/ml TNFα for 60 min. Following TNFα treatment, cells were collected and nuclear and cytoplasmic fractions separated. Lamin B served as the nuclear marker. Immunoblot is representative of immunoblots from two independent experiments. C, PS-1145 blocks phosphorylation of IkB. Cells were pretreated with 20uM PS-1145 for 2 h followed by treatment with 100ng/ml TNFα for 60 min. Following TNFα treatment, cells were collected, lysed and total protein probed for pIkB. Beta-actin served as a protein loading control. Immunoblot is representative of immunoblots from three independent experiments.