Figure 3.

Effect of TNF-α on PTX3 in the migration as well as invasion of gastric cancer cell lines. A. BGC-823 as well as SGC-7901 cells were transfected with PTX3 upregulation or a negative control (NC) plasmid. Western blotting as well as qRT-PCR of PTX3 upregulation efficiency. B. Representative images from cell migration and invasion assay after treated by rhTNF-α(20ng/ml) in PTX3 upregulation cells and NC cells. C. Western blotting investigation of PTX3, MMP-2, MMP-9 in PTX3 upregulation cells and NC cells after treated with rhTNF-α(20ng/ml) for 48h.