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. 2020 Feb 3;10(6):2859–2871. doi: 10.7150/thno.41279

Figure 4.

Figure 4

FGD1 interacts with PTEN to inhibit PTEN phosphates activity in osteosarcoma cells. A, the WCL of 293T cells were subjected to silver staining and mass spectrometry after transfected indicated plasmids. B-C, 293T cells transfected with indicated plasmids was harvested for co-immunoprecipitation. D-E, Western blotting analysis of WCL of U-2OS, MG63 and MNNG/HOS cells. F, a schematic diagram depicting the domain of PTEN. G, Flag-FGD1, Myc-PTEN-C and Myc-PTEN-N were translated in vitro, and the co-immunoprecipitation was performed to evaluate the interaction between the PTEN recombination protein and FGD1. H, a schematic diagram depicting FGD1 interacted with the N-terminal region of PTEN. I, U-2OS, MG63 and MNNG/HOS cells were transfected with indicated constructs. After 72 h, the spend medium of each treatment group were collected for release phosphatase assay. ***, P < 0.001. J, Western blotting analysis of the WCL of osteosarcoma tumor cells (U-2OS, MG63 and MNNG/HOS) after transfected with indicated siRNAs. K, Western blotting analysis of the WCL of osteosarcoma tumor cells (U-2OS, MG63 and MNNG/HOS) after transfected with indicated plasmids.