Figure 6.

The PAK6-SIRT4-ANT2 complex inhibits the apoptosis of prostate cancer cells. (A) The shRNA control/PAK6 in CWR22Rv1 cells was infected with or without His-ANT2, (B) the shRNA control/ANT2 in CWR22Rv1 cells was infected with or without GFP-PAK6, and equal amounts of protein lysates were harvested for western blot with antibodies, as indicated. (C-D) The flow cytometry detected the relevant changes in the cell cycle of different mutant CWR22RV1 cells, as indicated. The data are presented as a histogram of the mean ± standard error of the mean (SEM) of three independent experiments (Student's t-test). (E) The western blot analysis of the G2/M phase protein marker CyclinB1. (F) The CWR22RV1 cells transfected with different mutant ANT2 is shown after double staining with annexin V-FITC and propidium iodide. The dotted plot shows the annexin V-FITC in the X-axis and propidium iodide in the Y-axis (%). (G) CWR22RV1 cells were transfected with different mutant ANT2, and equal amounts of protein lysates were harvested for western blot with antibodies, as indicated. (H-J) Mice were subcutaneously injected with 1×10⁶of CWR22RV1cells with lentiviruses harboring shRNA, with or without the ANT2 mutant, and were grouped. The tumor volume was monitored over time, and the tumor was excised and weighed after 55 days.