Figure 4.

Circulation kinetics and biodistribution of CPEGAd in vivo. A qPCR quantification of virus genome copies (GCs) in blood at 2 or 10 min after intravenous administration of 1 × 10¹⁰ AdGFPLuc vp/animal in C57BL/6J mice of naked and CPEG-coated formulation (n = 5). B Luciferase activity quantification of protein extracts from liver, spleen, kidneys, lungs, and intestine of treated mice at five days after injection. C Bioluminescence in vivo imaging of mice at 5 days after injection. D Tumor-to-liver in vivo transduction study of CPEGAd complexes in PANC-1 tumor-bearing BALB/c Nu/Nu mice (n = 6) in each flank. Luciferase activity of protein extracts was quantified five days after the iv administration of 1 × 10¹⁰ vp/animal of naked AdGFPLuc and CPEGAd. *P < 0.05, **P < 0.01, ***P < 0.001