Figure 5.

Inflammatory response to GO-Collagen chamber in rat groin model. Hematoxylin-Eosin staining was performed to detect the formation of a fibrous capsule in different groups (A-C) [red boxes, between green dotted line]. CD 68 immunofluorescence showed that few macrophages assembled in the region of grafts in the GO-collagen chamber group, indicating high biocompatibility of the GO-collagen chamber (A-C, green boxes). Analysis of inflammatory-related gene expression (IL2, IL10, TNF-α) revealed that the GO-collagen chamber caused milder inflammatory reactions than the other two groups at both early (2 days) and later (20 days) stages (D-E).