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. 2020 Jan 22;10(6):2553–2570. doi: 10.7150/thno.41120

Figure 4.

Figure 4

MiR-1275 directly targets multiple antagonists of the Wnt/β-catenin and Notch signaling pathways. (A) Schematic of the predicted 3'-UTR wild-type and mutant reporter constructs of the target genes. (B) Construction of the designed dual-luciferase reporter plasmids. (C-G) Relative luciferase activities of the wild-type and mutant reporter plasmids in the A549, H1299, or PC9 cell lines after co-transfection with miR-1275-overexpressing cells or control cells. (H-I) Protein levels of DKK3, SFRP1, GSK3β, RUNX3, and NUMB in the three indicated cell lines were determined by Western blotting. Each experiment was performed three times. Data are shown as the mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001.