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. 2020 Feb 3;10(6):2727–2743. doi: 10.7150/thno.38729

Figure 1.

Figure 1

Differential distribution of acidic and lysosomal organelles in EGFR wild-type versus mutant NSCLC cell lines and tumors. A. Micrographs of EGFR wild-type (H1299 and H1666) and mutant (PC9 and H1650) NSCLC cells stained with acridine orange (green and orange) and lysotracker (blue). B. H1299 and PC9 cells were immunostained using antibodies specific for early endosomes (EEA1 and Rab5), late endosomes/lysosomes (Rab7 and LAMP1), and recycling endosomes (Rab11). The images represent maximum intensity projections using confocal microscopy. DAPI (blue) stains the nucleus. Scale bar: 10 μm. C. Immunohistochemical stains of LAMP1 and EGFR in primary human lung cancers. Staining demonstrates that the distribution of LAMP1 and EGFR (brown) in tumors closely resembles the distribution observed in NSCLC cell lines. Scale bar: 25 μm D. Western blot analysis of LAMP1 expression in NSCLC EGFR wild-type and mutant cell lines. Whole cell lysates were prepared, immunoblotted for early endosomes (EEA1), lysosomes (LAMP1) and loading controls (GAPDH and β-Actin).