Figure 7.

Phosphorylated YB-1 was enriched in the macrophage nuclei of ALF patients. (A, B) Macrophages in human liver paraffin section marked by CD64 antibody, and p-YB-1 expression were detected by immunofluorescence, and the p-YB-1 nuclear translocation positive macrophages were quantified (control n=5; ALF n=11). (C) Working model: LPS/GalN challenge decreased fecal and hepatic soyasaponin II levels. Soyasaponin II repressed YB-1 phosphorylation and nuclear translocation, p-YB-1 could translocate into nuclear and bind the promoter of Nlrp3, to promote Nlrp3 inflammasome priming, Il-1β overproduction and liver damage. Scale bars: 10 μm. *p<0.05.