Figure 4.

MRPS16 promotes tumor progression by the PI3K/AKT/Snail axis. A. Knockdown of MRPS16 decreased the protein levels of p-PI3K, p-AKT and Snail. B-C. Validation of over-expressing Snail in U-138MG and U-87MG cells by Western blot and qRT-PCR. D. Growth curves were tested by CCK-8 assay. E. Snail over-expression rescued the effects of MRPS16 knockdown in suppressing tumor cells growth. F. Over-expression of Snail rescued the effects of knockdown of MRPS16 in suppressing tumor cells colony formation. G - H. Transwell migration and invasion experiment was adopted as mentioned above. The numbers of migrating and invading cells are measured in the panel. Bars: 50μm. Statistical significance was tested using one-way ANOVA (Dunnett's tests) for multiple comparison and two-tailed t-tests. All the results are shown as the Mean ± Standard Deviation (SD) of three independent repeated experiments at least. **P < 0.01 and ***P < 0.001. None: non infected cells.