Figure 5.

MRPS16 over-expression increases Snail via PI3K/AKT pathway and binds with each other. A. U-138MG and U-87MG cells transfected with MRPS16 plasmid and co-cultured with PI3K/AKT signaling inhibitors were reaped, and the lysates were immune-blotted for P-p85, Tot.p85 and β-actin. B. U-138MG and U-87MG cells transfected with MRPS16 plasmid and co-cultured with PI3K/AKT signaling inhibitors were reaped, and the lysates were immune-blotted for p-AKT^Ser473, AKT and β-actin. C. U-138MG and U-87MG cells transfected with MRPS16 plasmid and co-cultured with PI3K/AKT signaling inhibitors were reaped, and the lysates were immune-blotted for Snail, Slug and GAPDH. D. Co-IP experiment indicated that MRPS16 could bind with Snail. Statistical significance was tested using one-way ANOVA (Dunnett's tests) for multiple comparison and two-tailed t-tests. * P < 0.05 and **P < 0.01.