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. 2020 Feb 3;11(8):2201–2212. doi: 10.7150/jca.40319

Figure 3.

Figure 3

miR-128 inhibited proliferation, EMT process and tumor growth. (A) Effect of miR-128 on cellular viabilities of C33A and HeLa were detected by CCK8. (B and C) The proliferation ability of C33A and HeLa cells transfected with pri-miR-128 or Anti-miR-128 were detected by colony formation and EdU assay. (D) Flow cytometric analysis showed that overexpression of miR-128 leads to an increase of the G0/G1 phase. (E) Flow cytometric assay showed overexpression of miR-128 promoted cell apoptosis in CC cells. (F and G) Transwell assays revealed that miR-128 suppressed invasion and migration ability in HeLa and C33A cells. (H) Western blot analysis of the indicated protein expression levels in HeLa cells following treatment with pri-miR-128 or Anti-miR-128 and the control groups. (I and J) miR-128 overexpression inhibited tumor growth rate and tumor weight following with the down-regulation of Ki67 using IHC. Experiments were performed 3 times, and data are presented as means ± SD.*P<0.05; **P<0.01; ***P<0.001; ns, not significant.