Figure 1.

Expression of Actinin-4 and actinin4-splice variant (Actn-4sv) in pancreatic tissue extracts from chronic pancreatitis (CP), pancreatic ductal adenocarcinoma (PDAC), pancreatic neuroendocrine neoplasia (pNEN), and normal tissue using western blot. A: Lanes 1-2: CP-, lanes 3-4: PDAC-, lanes 5-6: pNEN- and lanes 7-8: normal-pancreatic (CO) tissue extracts. B: Detection of Actn-4sv in three pNENs but not in the corresponding adjacent normal tissues (donor). Moderate to strong expression of Actn-4sv was detectable in all pNEN tissue extracts but not detectable in normal pancreatic tissue, CP and PDAC extracts. Using a pan-actinin-4 antibody, detecting both the wild type actinin-4 and Actn-4sv, positive staining was found in all lysates. Immunoblots for β-actin were used as loading control reference.