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. 2020 Feb 10;11(8):2318–2328. doi: 10.7150/jca.37503

Figure 2.

Figure 2

Expression of Actinin-4 and Actn4sv proteins in human PDAC cell lines and human pNEN cell line BON-1. A: Proteins were extracted from A549 cells that had been transfected (mock) with plasmid pEGFP (lane 1) or pEGFP-ACTN-4-SV (lane 2) and PDAC cell lines (lane 3-7) and blotted with 15H2 (Actn-4sv), 13G9 (pan-ACTN-4) and anti-β-actin (loading control) antibodies. B: Actn-4sv expression was only detectable in BON-1 cells. Lanes 1- 2: MiaPaca-2; lane 3-4: T3M4; lane 5: BON-1 (M); lane 6: BON-1after 72 h starvation (low glucose, serum free), lane 7, BON-1 (L). (L= low glucose 5.5µM and M = medium glucose 22 µM).