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. 2020 Feb 3;11(8):2222–2233. doi: 10.7150/jca.39679

Figure 1.

Figure 1

MiR-5094 directly targets STAT5b. (A) Alignment of wild-type seed sequence of the 3'-UTR of STAT5b mRNA (WT STAT5b 3'-UTR) and a mutated seed sequence of the miR-5094-binding site (Mut STAT5b 3'-UTR). The seed region is shown in bold. (B) Luciferase reporter assays. Luciferase reporter containing wild-type or mutant STAT5b 3'UTR was co-transfected with exogenous miR-5094 mimics (miR-5094) or negative mock control (NC) into HeLa cells. Luciferase activity was measured 24 h after transfection. Renilla luciferase activity was used to normalize the firefly luciferase activity. (C) MiR-5094 suppresses STAT5b mRNA expression in different cells at 24 h after transfection. The relative expression levels were normalized to same cells transient transfected with NC at same time point. (D) MiR-5094 suppresses STAT5b protein expression in different cells at 24 h after transfection. Mcs: miR-5094 mimics; inhibitor: miR-5094 inhibitor; si-1, si-2 and si-3: STAT5b siRNA. *P < 0.05 and **P < 0.01 represent the comparison with NC.