Skip to main content
. 2020 Feb 10;11(8):2303–2317. doi: 10.7150/jca.37242

Figure 2.

Figure 2

Baicalin treatment induced apoptosis in CRC cells via regulating apoptosis-related proteins. (A) FHC, RKO and HCT116 cells were treated with different concentrations of baicalin (0, 50 and 100 μg/mL) and 5-FU (25 μg/mL) for 48 h, and then cells were stained with DAPI and analyzed by fluorescence microscopy. (B) Cells which treated with 0, 50 and 100 μg/mL of baicalin and 25 μg/mL of 5-FU for 48 h were assayed for apoptosis by flow cytometry with Anexin V / propidium iodide double-staining. The percentage of apoptotic cells (including early and late apoptotic cells) in total cells were quantified and showed. *p < 0.05, **p < 0.01. (C) RKO and HCT116 cells were treated with different concentrations of baicalin (0, 50 and 100 μg/mL) for 48 h. The levels of apoptosis-related proteins (cleaved caspase-3, -8, -9, cleaved Parp-1, XIAP, NF-κB, Survivin, Bcl-2, Bax and p53) and β-actin (an internal control) were analyzed by western blot analysis. The relative protein levels in western-blot assay were quantified and marked under the protein bands.