Figure 3.

Baicalin treatment inhibited cell growth and decreased cell adhesion ability in CRC cells. (A) RKO and HCT116 cells treated with 0, 25, 50 and 100 μg/mL of baicalin were incubated for 4 days or 7 days, respectively; and then the colonies were stained and photographed with microscope under 10 × magnification. (B) The representative colonies were captured with microscope under 40 × magnifications. (C) RKO and HCT116 cells were cultured with the medium containing 0, 25, 50 and 100 μg/mL of baicalin in an incubator for 2 h and then adherent cells were stained and photographed with microscope. (D and E) The stained adherent cells were treated with 2% SDS buffer and the cell adhesion abilities were quantified by measuring the absorbance at 570 nm with a microplate reader and the adherence inhibition rates of cells were calculated. *p < 0.05, **p < 0.01, ***p < 0.001.