Figure 8.

Baicalin inhibited tumor growth and induced apoptosis in vivo by decreasing cell growth-related proteins and increasing apoptosis-related proteins. (A and B) Mice with orthotopically transplanted colon tumors were randomly divided into four groups and given intragastric administration every day for four weeks with negative control (NC), positive control (PC), low-dose baicalin (LD) and high-dose baicalin (HD) as described in “Materials and Methods”. The tumor size and mice weight were measured at 1, 4, 8, 11, 15, 18, 22, 26, 29 and 32 days and then presented in line charts. ***p < 0.001. (C) After tumor growth for 32 days, the mice were sacrificed and the orthotopically transplanted tumors were photographed by Inverted Fluorescence Microscope. (D) Tumors of each treatment group were isolated and weighted and plotted (a), *p < 0.05; and the tumor growth inhibition rate (%) in each treatment group was calculated and plotted (b). (E and F) Tumor tissues were dissected into two parts, one part was fixed with formaldehyde and embedded by paraffin for IHC experiments by using the specific antibodies against Ki67, CD44, CD133, E-Cadherin, N-Cadherin and Vimentin, respectively; another part was lysed with lysis buffer for western-blot experiments to assay the levels of CD44, CD133, E-Cadherin, N-Cadherin, Vimentin, Cyclin D1, Cyclin B1, c-Caspase 3, c-PARP-1 and β-Actin (as internal control), respectively.