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. Author manuscript; available in PMC: 2020 Mar 3.
Published in final edited form as: Methods Mol Biol. 2017;1658:3–22. doi: 10.1007/978-1-4939-7244-9_1

Fig. 1.

Fig. 1

Typical IMAC profile of mouse recombinant PrP purification. (a) IMAC profile. The peak of unbound proteins (stage a) typically reaches about 700 mAu. After this peak drops to the baseline (below 50 mAu, stage b), the IMAC buffer A (pH 8.0) is changed to the IMAC buffer B (pH 4.5). The PrP peak typically reaches approximately 600 mAu (stage c). Fractions with UV values above 50 mAu (stage d) are combined for subsequent purification. (b) Analysis of IMAC fractions in SDS-PAGE (10% bis-tris) following by silver staining. I, solubilized inclusion bodies; a, b, d, fractions collected at the stages a, b, and d, respectively