Figure 3.

CRMP4 promotes growth of injured sensory neurons. A, Immunoblot analysis of CRMP4 expression in sciatic nerve lysates of wild-type mice at different times following sciatic nerve transection. Samples collected after sciatic nerve transection span 0–3 mm distal, 0–3 mm proximal (proximal 1), or 3–6 mm proximal (proximal 2) to the injury site. Open arrowhead, CRMP4L (75 kDa); arrow, CRMP4S (65 kDa); solid arrowhead, tCRMP4 (55 kDa). The data are representative of results obtained from seven mice. B, C, Representative pictures (B) and quantification (C) of the regrowth of dissociated E15-16 wild-type or Crmp4^−/− DRG neurons plated in microfluidic devices at 24 h after axotomy indicated an impaired regrowth after axotomy in the Crmp4^−/− DRG neurons. Data are represented as the mean neurite outgrowth per channel ± SEM (n = 7–8, two-tailed Student t test). Scale bar, 200 μm.