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. 2020 Mar 2;7(2):ENEURO.0479-19.2020. doi: 10.1523/ENEURO.0479-19.2020

Figure 5.

Figure 5.

Calpain-dependent cleavage of CRMP4 occurs in degenerating axons following injury. A, B, Immunoblot analysis of the calpain substrate fodrin in sciatic nerve (A) and optic nerve (B) lysates collected at the indicated DPI. The arrowheads indicate the presence of fodrin breakdown products, which are present when calpain is active. C, Western immunoblot analysis of CRMP4 expression in lysates prepared from P4 to P7 rat DRG neurons and treated with calpain in vitro. D, E, Western immunoblot analysis of CRMP4 expression in axonal lysates prepared from wild-type (D) or Caspase-3−/− (E) E12.5 DRG explants grown on cell-filter inserts collected at 3 h after axotomy. DRG explants were treated with ALLN to inhibit calpain. F, Western immunoblot analysis of CRMP4 expression in lysates prepared from P4 to P7 rat DRG neurons that were treated for 5 h with either GST or GST-Nogo-22. Arrow, CRMP4S (65 kDa); solid arrowhead, tCRMP4 (55 kDa).