Fig. 5.

EN2 suppresses cell migration/invasion in glioma cells. a and b Wound healing assays demonstrate that EN2 overexpression inhibits the migration of U251 cells (n = 4). The migration of cells into the wound was assessed at 0, 24 h and 48 h. Scale bar, 200 μm. c and d EN2 overexpression reduces the rate of migration of U251 cells by Transwell migration assays (n = 4). Scale bar, 200 μm. e and f EN2 overexpression decreases the invasive ability of U251 cells by Transwell invasion assays (n = 5). Scale bar, 200 μm. g and h The level of MMP-9 protein are downregulated by EN2 overexpression. i MMP-9 mRNA is upregulated by EN2 overexpression (n = 3). j MG132 rescues MMP-9 proteins in EN2 overexpressed cells. k A schematic demonstrates that EN2 regulates cell proliferation/apoptosis/invasion of glioma cells