Table 5.
Primers used for real-time quantitative PCR
| Target genes | Primer Sequences(5′~ 3′) | Tm (°C) | GenBank Accession No. |
|---|---|---|---|
| SCD1 |
F: TCCTGTTGTTGTGCTTCATCC R: GGCATAACGGAATAAGGTGGC |
59 | AY241933 |
| FAS |
F: GCAAAGTGGTCATTCAGGTACG R: CCCAGTGATGATGTAGCTCTTG |
60 | KM980092.1 |
| PPARG |
F: CAGTGTCTGCAAGGACCTCA R: GTAAAAGGCATGGGAGTGAT |
59 | HG270143.1 |
| SREBP1 |
F: CTGACGACCGTGAAAACAGA R: AGACGGCAGATTTATTCAACTT |
58 | KU517672.1 |
| GPAT |
F: GCAGGTTTATCCAGTATGGCATT R: GGACTGATATCTTCCTGATCATCTTG |
59 | AY515690.1 |
| AGPAT6 |
F: AAGCAAGTTGCCCATCCTCA R: AAACTGTGGCTCCAATTTCGA |
59 | DY208485 |
| ACACA |
F: TCCTGCTGCTATCGCTACTCCA R: CGCACTCACATAACCAACCAT |
61 | DQ773054.1 |
| GAPDH |
F: TGGAAAGGCCATCACCATCT R:CCCACTTGATGTTGGCAG |
60 | NM001034034.1 |