Table 1.

PEI transfection to produce rAAV in 6-well plate

AAV double transfection		AAV triple transfection		PEI solution
Transgene plasmid	675 ng	Transgene plasmid	675 ng	PEI	30 μl
Capsid-helper plasmid	2025 ng	Capsid-helper plasmid	675 ng	1.5 M NaCl	10 μl
1.5 M NaCl	10 μl	Adeno helper plasmid	1350 ng	Water	60 μl
Water	to 100 μl	1.5 M NaCl	10 μl	Total	100 μl
Total	100 μl	Water	to 100 μl
		Total	100 μl