Table 3.

PEI Transfection to produce concentrated rAAV preparations

AAV double transfection		AAV triple transfection		PEI solution
Transgene plasmid	12.5 μg	Transgene plasmid	12.5 μg	PEI	0.5 mL
Capsid-helper plasmid	37.5 μg	Capsid-helper plasmid	25 μg	1.5 M NaCl	0.1 mL
1.5 M NaCl	0.1 mL	Adeno helper plasmid	12.5 μg	Water	0.4 mL
Water	to 1 mL	1.5 M NaCl	0.1 mL	Total	1 mL
Total	1 mL	Water	to 1 mL
		Total	1 mL