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. 2020 Mar 2;19:53. doi: 10.1186/s12934-020-01316-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Immune protection conferred by S.cerevisiae EBY100/pYD5-HA against lethal H7N9 virus challenge. Mice were intranasally challenged with a lethal dose (10 × LD₅₀) of A/Anhui/1/2013 (AH-H7N9) virus at 2 weeks after the final immunization (n = 10/group). a Weight change as a percentage. Bars indicate SDs. b Lung viral titers were determined by a plaque assay at day 3 after challenge (n = 3 of 10 challenged mice). c Survival rate. Asterisk indicates significant difference compared to S.cerevisiae EBY100/pYD5 and PBS groups (p < 0.05)