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. Author manuscript; available in PMC: 2020 Mar 3.
Published in final edited form as: Cell Rep. 2019 Feb 12;26(7):1800–1814.e5. doi: 10.1016/j.celrep.2019.01.075

Figure 2. siRNA Screen Uncovers MARCH8 as an E3 Ligase Required for HCV Infection.

Figure 2.

HCV (pFL-J6/JFH(p7-Rluc2A)) infection (multiplicity of infection [MOI] of 0.1) relative to NT control after siRNA-mediated knockdown of E3 ligases identified as NS2 interactors in the HT-GPCAs screen, measured via luciferase assays at 72 hr after infection in Huh7.5.1 cells and normalized to cell viability. Data are pooled from two independent experiments with 8 replicates each. Shown are means ± SD; *p < 0.05, **p < 0.01, ***p < 0.001 relative to NT control by 1-way ANOVA followed by Dunnett’s post hoc test.