Figure 2.

The MRK hydrogel induces ICD and facilitates intracellular uptake of loaded cargo in vitro. (A, B) Flow cytometry measurement of ROS induced by PBS, KN93, MR52, or MRK hydrogel. Data are presented as the mean ± SEM (n = 4). (C) ATP levels in the B16F10 cells treated with indicated compounds were measured using the multiscan spectrum. Data are presented as mean ± SEM (n = 4). (D, E) Flow cytometry analyses of CRT expression on the cell membrane surface. Data are presented as the mean ± SEM (n = 4). (F) Representative immunofluorescence staining images of CRT expression (green) on the surface of B16F10 cells after various treatments. Phalloidine-rhodamine (red) indicates the cytoskeleton (scale bar, 20 µm). (G) Representative immunofluorescence images of the intake of Cy5 in B16F10 cells treated with MR₅₂-Cy5 or free Cy5 at the indicated time points (scale bar, 50 µm). (H, I) Flow cytometry analyses of Cy5 uptake in B16F10 cells after MR₅₂-Cy5 or free Cy5 treatments at indicated time points. Data are presented as the mean ± SEM (n = 3).