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. 2020 Feb 10;10(7):3049–3063. doi: 10.7150/thno.42385

Figure 5.

Figure 5

Combining the MRK hydrogel and the PD-1 antibody exerted synergistic antitumor effects. (A, B) Detection of PD-L1 expression levels in BMDMs after various treatments, as indicated by flow cytometry. Data are presented as the mean ± SEM (n = 4). (C) Schematic illustration of the combination of the MRK hydrogel and PD-1 antibody treatment schedule for subcutaneously implanted B16F10 melanoma model. (D) Tumor growth monitored for 12 days in mice after various treatments, as indicated. Data are presented as the mean ± SEM (n = 8). (E) The long-term survival in the corresponding treatment groups recorded according to the Kaplan-Meier analysis (n = 8). (F) Survival curves for the treated group (receiving three stitches of the MRK hydrogel and four stitches of the PD-1 antibody treatment) and PBS group (n = 9) in the B16F10 melanoma model. (G) Survival curves for the treated and controlled mice, as indicated (n = 12) in the B16-luc melanoma model. (H) Tumor growth monitored in C57BL/6 mice (n = 9-10) with B16-luc melanoma that were treated with anti-CD8 neutralizing antibody and/or MRK plus anti-PD-1 for 12 days. (I) Survival curves for the corresponding treatment groups according to the Kaplan-Meier analysis (n = 9-10).