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. 2020 Feb 10;10(7):3351–3365. doi: 10.7150/thno.41219

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ATRX expression is increased via DNA demethylation mediated by STAT5b/TET2 complex. (A) ATRX expression of xenograft gliomas formed by LN229R and LN229 cells. Scale: 20 μm. Schematic used elements from Servier Medical Art: https://smart.servier.com. (B) ATRX expression in HG7, HG9, LN229, HG7R, HG9R and LN229R detected by IF. Scale: 20 μm. (C) Expression of γ-H2AX in ATRX express and ATRX loss glioma tissues. Scale: 20 μm. ***P < 0.001, Chi-squared test. (D) Heatmap with the differentially expressed genes annotated with six gene families. (E) Co-IP assay of STAT5b and TET2 in HG7R and HG9R cells treated with STAT5b knockdown. (F) ChIP-PCR assay showing the TET2 enrichment in ATRX promoter regions of HG7R and HG9R cells treated with STAT5b siRNAs. Error bars indicate mean ± SD. (G-H) The average levels of methylation in ATRX promoter between STAT5b/TET2 knockdown LN229R group and NC group. ***P < 0.001, Student's t-test.