Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 25;8:92. doi: 10.3389/fcell.2020.00092

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Dennis, Splawn and Kean.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Combinations of chromium, vitamins B12, E, and K in supplemented media at day 21. Vitamins and minerals (chromium, vitamins B12, E, and K) which had been identified as promoting COL2A1 promoter-driven expression in the initial screen were tested alone and in combination in supplemented medias 1 (A) Basal media + TGFβ1; 2 (B) Basal + TGFβ1 + biotin + iodine; 3 (C) Basal + TGFβ1 + copper; 4 (D) Basal + TGFβ1 + iodine + thyroxine + copper. Vitamins and minerals were supplemented at their individually optimized concentration in basal media (2.8 μg/L, 91.4 ng/L, 1.84 μg/L, and 2.2 μg/L, respectively). The values are normalized to the plate control (basal media); the red dashed red line indicates the luminescence achieved with that particular supplemented media alone. Green circles indicate values that are not significantly different to supplemented media (red dashed line), all are greater than basal media. Conditions that were statistically greater than their respective supplemented media are represented by an orange triangle () and an *, black symbols (▼) indicate that those values were lower than the respective supplemented media. n ≥ 3 ± S.D.

Inline graphic — Combinations of chromium, vitamins B12, E, and K in supplemented media at day 21. Vitamins and minerals (chromium, vitamins B12, E, and K) which had been identified as promoting COL2A1 promoter-driven expression in the initial screen were tested alone and in combination in supplemented medias 1 (A) Basal media + TGFβ1; 2 (B) Basal + TGFβ1 + biotin + iodine; 3 (C) Basal + TGFβ1 + copper; 4 (D) Basal + TGFβ1 + iodine + thyroxine + copper. Vitamins and minerals were supplemented at their individually optimized concentration in basal media (2.8 μg/L, 91.4 ng/L, 1.84 μg/L, and 2.2 μg/L, respectively). The values are normalized to the plate control (basal media); the red dashed red line indicates the luminescence achieved with that particular supplemented media alone. Green circles indicate values that are not significantly different to supplemented media (red dashed line), all are greater than basal media. Conditions that were statistically greater than their respective supplemented media are represented by an orange triangle () and an *, black symbols (▼) indicate that those values were lower than the respective supplemented media. n ≥ 3 ± S.D.