Figure 6.

Hv1 and NADPH component activation is altered with age. A‐G, Western blot analysis revealed significant increases in Hv1 and NADPH oxidase subunits Gp91phox, P22phox, P67phox, and P47phox protein expression in aged sham compared to adult mice. H, Representative immunofluorescent images of the hippocampal dentate gyrus showing that Hv1‐positive cells co‐expressed with CD16/32 were increased in aged brain after surgery (scale bar, 50 μm). Brain sections were stained with Hv1 (red) and CD16/32 (green). All data are presented as the mean ± SEM. n = 4‐5; *P < .05, **P < .01, ***P < .001 vs adult sham, Student's t test