Table 2.

Multiple linear regression between ACSL4 mRNA expression (dependent variable) and other variables. The ACSL4 mRNA expression was measured based on ACTB in tumor and adjacent normal tissues with 2-^ΔΔct from at least 2 experiments. Estrogen and progesterone receptors and human epidermal growth factor receptor-2 expressions were measured using immunohistochemistry (IHC) method, and the results are reported as expression of positive (+) or negative (-). Ki-67 nuclear expression was measured using IHC method, and the results are reported as percent of expression. p53 expression was measured based on IHC, and the results were reported as the mutant p53 (+) and wild-type p53 (-). * P ≤ 0.05 indicate significant differences between variables and expression of ACSL4 group. The following linear regression (backward) equation was used for calculating the mRNA expression of gene of interest; ACSL4 mRNA expression = 0.386 p35 - 0.560 ki - 67 - 0.416 age

ACSL4 mRNA expression		Unstandardized coefficients	Standardized coefficients	P values
Models	Variables	B	Beta
1	Constant	1.796		0.001
	age	-0.012	-0.410	0.019*
	grade	0.111	0.186	0.308
	stage	-0.014	-0.020	0.912
	tumor size	0.000	0.000	0.998
	Estrogen receptors	0.012	0.013	0.962
	Progesterone receptors	-0.040	-0.049	0.837
	human epidermal growth factor	0.030	0.097	0.583
	Ki67	-0.017	-0.679	0.003*
	p53	0.252	0.343	0.056
6	Constant	1.945		0.000
	age	-0.012	-0.416	0.006*
	Ki67	-0.014	-0.560	0.001*
	p53	0.284	0.386	0.014*