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. 2020 Feb 4;17(4):498–509. doi: 10.7150/ijms.37833

Figure 6.

Figure 6

The secretion of cytokines induced by S100A8 and S100A9 in BEAS-2B cells inhibits asthmatic neutrophil apoptosis. (A) BEAS-2B cells were incubated with or without 10 μg/mL of S100A8 and S100A9 for 48 h. The supernatant (Sup) was collected and added to the fresh neutrophils isolated from the peripheral blood of asthmatic subjects (n=5). (B) Asthmatic neutrophils or neutrophils and BEAS-2B cells (1:1) were incubated for 24 h in the absence or presence of 10 μg/mL S100A8 (left panel) and S100A9 (right panel). Apoptosis of asthmatic neutrophils was analyzed by measuring the binding of annexin V-FITC and PI. Data are presented relative to the control set at 100%, as the means ± SD. *p < 0.05 and **p < 0.01 indicates a significant difference between the control and S100A8- or S100A9-treated groups, or between the control supernatant and supernatant-treated groups.