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. 2020 Feb 10;16(7):1264–1273. doi: 10.7150/ijbs.38415

Figure 3.

Figure 3

SASH1 mutation down-regulated SASH1 expression in PIG1 cells. (A) The c.1761C>G point mutation of the construct pcDNA3-SASH1 and SASH1 c.1519T>G mutant was confirmed by sequencing. (B) Bright field and green fluorescence field showed the point mutant in PIG1 cells after incubation for 72 h with a lentiviral RNAi vector. (C) SASH1 mRNA expression was down-regulated by 38.7% and 28.6% in S587R-SASH1 PIG1 cells, as compared to WT-SASH1 and S507A-SASH1 PIG1 cells. (D) Western blot analysis indicated that SASH1 protein expression of S587R-SASH1 PIG1 cell was down-regulated by 50.6% and 38.1%, compared with WT-SASH1 and S507A-SASH1 cells.