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SASH1 mutations down-regulated THBS1 expression. (A) qPCR showed mRNA expression of THBS1 was reduced by 63.3% and 42.5% in S587R-SASH1 PIG1 cell, compared to the WT-SASH1 and S507A-SASH1 PIG1 cells. (B) Western blot revealed THBS1 protein was reduced by 74.2% and 48.5% in S587R-SASH1 PIG1 cells compared with WT-SASH1 and S507A-SASH1 PIG1 cells.
