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. 2020 Feb 10;16(7):1252–1263. doi: 10.7150/ijbs.38835

Figure 3.

Figure 3

EHMT2 inhibition or knockdown accelerates RAVSMCs autophagy. A. The RAVSMCs were fluorescence staining with α-SMA after treated with 5 μM of BIX01294 or DMSO for 48h (n=3), red arrows indicated vacuoles in VSMCs. B. The representative images of autophagic vacuole under a transmission electron microscope and yellow arrow indicates autophagic vacuole in RAVSMCs with 5 μM of BIX01294 or DMSO stimulation for 12h (scale bar, 1 μm). C. LC3 and histone H3 protein levels were detected by western blot in RAVSMCs treated with 5 μM of BIX01294 for different times (n=4). D. The protein levels of LC3 and histone H3 were assessed by western blot in RAVSMCs treated with indicated stimulations (n=4). E. The mCherry-GFP-LC3 was overexpressed in the RAVSMCs, which were subsequently stimulated with 5 μM of BIX01294 or DMSO for 6 hours. Yellow and red indicate autophagosomes or autolysosomes, respectively (n=3). F. The protein levels of LC3 and histone H3 were assessed by western blot in RAVSMCs infected with lenti-shRNA or lenti-shEHMT2 (n=4). G. Western blot was used to evaluate the protein levels of LC3 and histone H3 in RAVSMCs treated with indicated stimulations for 8h (n=4). Histone H3 serves as a loading control.