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. 2020 Feb 10;16(7):1238–1251. doi: 10.7150/ijbs.39768

Figure 2.

Figure 2

Axl-148b conjugate affects ALCAM and ITGA5 expression in tumor cells. (A-D) Western blot analysis of ALCAM and ITGA5 protein expression for the indicated AXL+ (A-C) or AXL- (D) tumor cells left untreated (controls=ctrl) or treated with 400 nmol/L of axl, axl-148b or scr-148b aptamers. Alternatively, cells were transfected with 75 nmol/L of miR-148b precursors (pre-148b) or its negative control (pre-control). Filters were first stained for ALCAM, stripped and reprobed with anti-ITGA5 antibodies. Protein modulations were calculated relative to axl or controls (pre-ctrl), normalized on loading controls (GAPDH or actin) and expressed as percentages (%). At least 2 independent experiments were performed and representative results are shown. scr= scramble.